Инкапсулированные генетически модифицированные клетки СНО-К1 как источник рекомбинантного FGF2 человека

Abstract

The aim of the investigation was to obtain the transgenic mammalian cell line CHO-K1 that expresses recombinant human FGF2 and secretes it into cultural medium and to compare the abilities of a monolyer cell culture and the cells encapsulated in alginate microcapsules to produce and to secrete this protein. Methods. The non-viral gene transfer method, RT-PCR, Western blot analysis were used for the investigation. Results. The expression vector pC1-F contained the recombinant human FGF2 had been constructed. This vector was used for the CHO-K1 cells transfection. As a result, a stable transgenic cell line expressing FGF2 was obtained. A few positive signals were detected via Western blot analysis of the conditioned cultural media. The same protein products were revealed in a case of the conditioned cultural media where alginate microcapsules with the transgenic cells had been cultivated. Conclusion. Thus the obtained genetically modified CHO-K1 cells remain a sourсe of the recombinant human FGF2 after their encapsulation in alginate microcapsules.

Key words: recombinant human FGF2, transfection, encapsulation, alginate microcapsule.