Вплив делеції гена β-катеніну на морфологію та фізіологію кардіоміоцитів за умов дії стимуляторів гіпертрофії

Abstract

Aim. The investigation of β-catenin signaling function during cardiomyocyte hypertrophy development under the one allele of β-catenin conditional cardiospecific knock-out condition was the aim of present work. Methods. The heart of new-born trangenic and wild-type animals were used for the primary culture isolation. The physiological activity was evaluated with MTT-test. The primary cardiomyocytes were H&E stained and morphological parameters were measured. Results. The physiological activity elevation was detected for wild-type and haploinsufficient animals after lithium chloride treatment comparing to control cells in MTT-test. The inhibition of physiological activity was detected for all genotypes comparing to controls. The binucleated cells distribution was shown to differ between the control and LiCl-treated cells. Also the frequency of binucleated cells tended to increase for mutants comparing to controls after canonical Wnt stimulation with LiCl. Conclusions. The deletion of β-catenin leads to disruptions in cell morphology and physiology in postnatal cardiovyocytes and attenuated cell hypertrophy development possibly due to canonical Wntsignaling inhibition. Keywords: hypertrophic stimuli, β-catenin haploinsufficiency, β-catenin deletion, canonical Wnt-signaling.