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dc.contributor.author |
Шаптуренко, М.Н. |
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dc.contributor.author |
Печковская, Т.В. |
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dc.contributor.author |
Вакула, С.И. |
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dc.contributor.author |
Хотылева, Л.В. |
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dc.date.accessioned |
2021-02-15T13:50:10Z |
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dc.date.available |
2021-02-15T13:50:10Z |
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dc.date.issued |
2015 |
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dc.identifier.citation |
Сравнительный анализ аллельного состава микросателлитных локусов Brassica oleracea var. capitata L. / М.Н. Шаптуренко, Т.В. Печковская, С.И. Вакула, Л.В. Хотылева // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 170-173. — Бібліогр.: 8 назв. — рос. |
uk_UA |
dc.identifier.issn |
2219-3782 |
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dc.identifier.uri |
http://dspace.nbuv.gov.ua/handle/123456789/177381 |
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dc.description.abstract |
Aims. White cabbage is characterized by high level of intraspecifi c heterogeneity due to some biological features that
causes diffi culties for breeding at creation constant form and maintaining of their genetic typicality. Thus, we studied
the effectiveness the set of SSR markers for both white cabbage diversity analysis and pure lines genotyping. Methods.
Molecular-genetic studies were performed trough SSR PCR. Effectiveness the set of markers was evaluated by counting
allelic richness, PICs, heterogeneity, allele frequencies using GenAlEx 6.5. To determine B.o.var capitata diversity with
low and high variations, Jaccard dissimilarity index was estimated by Darwin software. Results. 15 microsatellite loci
were characterized for the information content, allelic frequencies and heterogeneity level. The effective multiallelic
markers (Bo20TR, BoDCTD4, BoPC34, BoPLD1, BoCalc) with high information content (PIC > 0.7) that could be
successfully used for the analysis of inter- and intravarietal polymorphism B. oleracea var.capitata were defi ned. Based
on the SSR-evaluation and subsequent clustering the genetic structure of breeding collection was established, which
showed that most experimental forms have a common ancestral genetic basis, in spite of different origins. Conclusions.
Proposed set of informative SSR markers is a useful tool for the selection of genetically constant material, as well as
divergent cross combinations for the hybrid breeding. Identifi ed donors of rare SSR alleles potentially could be a source
of valuable genetic segregation for further B. oleracea improvement.
Keywords: Brassica oleracea var. capitata L. f. alba DC, genetic diversity, SSR. |
uk_UA |
dc.description.sponsorship |
Выражаем благодарность сотрудникам РУП Институт овощеводства НАН Беларуси за предоставленный для исследований селекционный материал капусты белокочанной. |
uk_UA |
dc.language.iso |
ru |
uk_UA |
dc.publisher |
Інститут молекулярної біології і генетики НАН України |
uk_UA |
dc.relation.ispartof |
Фактори експериментальної еволюції організмів |
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dc.subject |
Прикладна генетика і селекція |
uk_UA |
dc.title |
Сравнительный анализ аллельного состава микросателлитных локусов Brassica oleracea var. capitata L. |
uk_UA |
dc.title.alternative |
Comparative diversity simple sequence repeat loci from Brassica oleracea var. capitata L. |
uk_UA |
dc.type |
Article |
uk_UA |
dc.status |
published earlier |
uk_UA |
dc.identifier.udc |
635.342:57.015.3:577.21 |
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