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періодичних видань НАН України
періодичних видань НАН України
Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast
Репозиторій DSpace/Manakin
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Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast
Тема:
Original contributions
Посилання:
Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast / M. Jia, S. Souchelnytskyi // Experimental Oncology. — 2010. — Т. 32, № 3. — С. 209-212. — Бібліогр.: 12 назв. — англ.
Підтримка:
We are grateful to Carina Hellberg for advice on immunohistochemistry. This work is supported in part by grants from the programmes of the Dept. of Oncology-Pathology, Karolinska Institutet, Ludwig Institute for Cancer Research, the Swedish Cancer Society, the Swedish Research Council, the Swedish Institute, the EU program RTN «EpiPlastCarcinoma», INTAS, UICC/American Cancer Society, Hiroshima University and Merck KGaA to S.S.
Дата:
2010
Переглядів:
592
Завантажень:
214
Анотація:
Aim: To study the expression of Kinase Suppressor of Ras 2 (KSR2) in human breast tumors and its effect on proliferation of breast epithelial cells. We reported previously that KSR2 was up-regulated in immortalized human breast epithelial cells. Methods: Proteomics technologies, systems biology tool for a KSR2 network analysis, immunoblotting, siRNA technology, overexpression of KSR2, cell proliferation assays and immunohistochemistry of tissue microarray of human breast tumors and normal breast tissue were used. Results: In conditionally immortalized primary epithelial cells KSR2 expression was shown to be up-regulated. The involvement of KSR2 in regulation of cell proliferation was predicted by a KSR2-centered network analysis. We observed that KSR2 down-regulation with specific siRNA inhibited cell proliferation. By immunohistochemistry of tissue microarray it was demon strated that KSR2 expression was enhanced in human invasive breast carcinomas. Conclusion: Our findings propose KSR2 as a new marker of immortalization, which has an impact on cell proliferation.
