http://dspace.nbuv.gov.ua:80/handle/123456789/32293 Sat, 18 Apr 2026 16:19:09 GMT 2026-04-18T16:19:09Z http://dspace.nbuv.gov.ua:80/bitstream/id/92143/ http://dspace.nbuv.gov.ua:80/handle/123456789/32293 http://dspace.nbuv.gov.ua:80/handle/123456789/138612 R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine — 50th Anniversary Kavetsky, R.E. Fri, 01 Jan 2010 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138612 2010-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138611 Age-related function of tumor suppressor gene TP53:contribution to cancer risk and progression Cherdyntseva, N.V.; Gervas, P.A.; Litvyakov, N.V.; Stakcheeva, M.N.; Ponomaryeva, A.A.; Dobrodeev, A.Yu.; Denisov, E.V.; Belyavskaya, V.A.; Choinzonov, E.L. Aim: To examine the influence of combined genotypes of TP53 (exon 4, intron 3, intron 6) and XRCC1 (codon 10) on lung cancer age of onset. Methods: TP53 polymorphisms in codon 72 of exon 4 (Arg72Pro), in intron 3 (16 bp duplication), in intron 6 (G/A transition) and XRCC1 polymorphism in codon 10 (Arg399Gln) were analyzed in blood cells of 177 lung cancer patients and 196 healthy donors with Restriction Fragment Lenth Polymorphism PCR. Results: We showed that combination of TP53 variant genotypes and XRCC1 variant genotype is associated with the increased lung cancer risk in younger, but not elderly, smokers. In contrast, wild allele combination increases lung cancer risk for individuals over the age of 60. Conclusion: Our data confirm antagonistic pleiotropy hypothesis indicating that p53 protects the organism against cancer early in life, but promoting aging phenotype, including late life cancer in older persons. Fri, 01 Jan 2010 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138611 2010-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138610 Immunocytochemical markers in acute leukaemias diagnosis Gluzman, D.F.; Nadgornaya, V.A.; Sklyarenko, L.M.; Ivanovskaya, T.S.; Poludnenko, L.Yu.; Ukrainskaya, N.I. The study included 1742 patients with acute myeloblastic leukaemias (AML) and acute lymphoblastic leukaemias (ALL), Kyiv city residents and patients from 20 regions of Ukraine. Bone marrow and blood smears were sent at diagnosis to Reference Center. The analysis was based on May-Grünvald-Giemza (MGG) stain and cytochemical reactions (MPO, acNSE, CAE, AP, PAS). Immunocytochemical techniques (APAAP, LSAB) and broad panel of monoclonal antibodies (MoAbs) against lineage specific and differentiation antigens of leukocytes were employed for immunophenotyping of leukemic blast cells directly in blood and bone marrow smears. Different types of AML were defined by the expression of the cell surface and cytoplasmic antigens. Immunocytochemical study was required especially in diagnosing of AML with minimal differentiation, acute megakaryoblastic leukaemia, acute erythroid leukaemia and acute leukaemias of ambiguous lineage. Acute lymphoblastic leukaemias was broadly classified into B-lineage and T-lineage ALL. According to the degree of B-lymphoid differentiation of the blast cells four subtypes of B-lineage ALL were established. T-lineage ALL observed in patients were also divided into four subtypes. Immunocytochemical examination was required to diagnose AL of ambiguous lineage with no clear evidence of lineage differentiation (acute undifferentiated leukaemia) or those with blasts that express markers of more than one lineage (mixed phenotype acute leukaemias). Fri, 01 Jan 2010 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138610 2010-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138609 Radiation induced thyroid cancer: fundamental and applied aspects Tronko, M.; Bogdanova, T.; Voskoboynyk, L.; Zurnadzhy, L.; Shpak, V.; Gulak, L. Aim: To describe the epidemiology and pathology of thyroid cancer in Ukraine, and to perform the molecular analysis of genetic alterations more frequently found to be associated to papillary carcinomas (PTC) in a selected group of PTC. Materials and Methods: Relationship between the thyroid cancer incidence and gender, age, and place of residence of subjects aged 0–18 years at the time of the Chernobyl accident (5427 subjects of thyroid cancer, among which 3996 (73.6%) were children aged 0 to 14 years at the time of the accident, and 1431 (26.3%) were adolescents aged 15 to 18 years was studied. Pathologically analyzed thyroid carcinomas were obtained from 640 patients (20–40 years old at the time of surgery and born before the Chernobyl accident), and from 90 patients (11–22 years old at the time of surgery and born after the accident). All patients were operated during 2006–2008. RET/PTC rearrangements and BRAFV600E mutation were analyzed in 35 cases of PTC. Results: A comparison between the thyroid cancer incidence rates in the 6 highest contaminated regions of Ukraine and in the other 21 regions shows the most significant difference between the rates for the last three years of follow-up, which confirms that a direct relationship is still present between the rise in thyroid cancer incidence and the post Chernobyl radiation exposure. Much lower incidence of thyroid cancer in subjects, who were born after the accident, additionally confirmed a direct relationship between the Chernobyl accident and thyroid cancer development at least in those who were aged up to 18 years at the time of the nuclear accident. Pathological results showed that with increasing latency the decrease has been noted in the percentage of PTC with solid structure, a decrease in invasive properties of tumors, as well as an increase in the percentage of PTC with papillary-follicular structure, encapsulated forms, and «small» carcinomas measuring up to 1 cm. Molecular-biological studies of PTC revealed more common RET/PTC1 and RET/PTC3 rearrangements (34.3% of cases), than BRAFV600E mutation (24%cases). Conclusion: After 22 years from the Chernobyl nuclear accident the number and incidence of thyroid cancer cases in Ukraine was steadily increased in the cohort of those who were children and adolescents at the time of the accident. Most common thyroid tumors (PTC) were characterized by significant changes in histological structure with increasing latency. PTC with any RET/PTC rearrangements had more aggressive behavior than BRAFV600E-positive tumors or PTC without gene alterations. Fri, 01 Jan 2010 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138609 2010-01-01T00:00:00Z