http://dspace.nbuv.gov.ua:80/handle/123456789/133170 Tue, 21 Apr 2026 13:27:52 GMT 2026-04-21T13:27:52Z http://dspace.nbuv.gov.ua:80/bitstream/id/395864/ http://dspace.nbuv.gov.ua:80/handle/123456789/133170 http://dspace.nbuv.gov.ua:80/handle/123456789/138003 Radioprotective properties of sodium humate in radiation-induced mutagenesis in cultured lymphocytes of thyroid cancer patients Shkarupa, V.M.; Klymenko, S.V. Aim: To investigate the effect of sodium humate on the level of cytogenetic damage in culture of lymphocytes of patients with thyroid cancer after γ-irradiation. Materials and Methods: Metaphase analysis of chromosome aberrations in cultured peripheral blood lymphocytes of 10 individuals with thyroid cancer was performed after irradiation of lymphocytes in vitro at a dose of 1 Gy from ¹³⁷Cs source at the early G₀ phase of cell cycle. Sodium humate was added to cell culture for 30 ± 15 min after phytohemagglutinin stimulation at concentrations of 10 and 100 μg/ml. Results: Sodium humate exhibited antimutagenic properties. The preparation at a concentration of 10 μg/ml was more effective than at a concentration of 100 μg/ml, reducing the average incidence of radiation-induced chromosome aberrations by 51.88 and 38.77%, respectively. The most pronounced antimutagenic effect of sodium humate was the reduction of the frequency of chromosomal type aberrations, however, such efficiency varied between individual patients with thyroid cancer. Conclusions: Sodium humate could be considered as a potential therapeutic modifier of radiation damage. Fri, 01 Jan 2016 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138003 2016-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138002 Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation Franskevych, D.V.; Grynyuk, I.I.; Prylutska, S.V.; Pasichnyk, G.V.; Petukhov, D.M.; Drobot, L.B.; Matyshevska, O.P.; Ritter, U. Aim: To estimate the combined action of C₆₀ fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm²) was used for C₆₀ fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C₆₀ fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C₆₀ fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G₁ phase of cell cycle was observed after C₆₀ fullerene photoexcitation. Conclusion: Photoexcited C₆₀ fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells. Fri, 01 Jan 2016 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138002 2016-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138001 Detection of notch1 c.7544_7545deICT mutation in chronic lymphocytic leukemia using conventional and real-time polymerase chain reaction Bilous, N.I.; Abramenko, I.V.; Chumak, A.A.; Dyagil, I.S.; Martina, Z.V. Aim: To evaluate real-time polymerase chain reaction (PCR) assay system for detection of NOTCH1 c.7541_754delCT mutation in chronic lymphocytic leukemia (CLL) patients. Material and Methods: A total of 325 CLL patients were included in the study. Screening for NOTCH1 c.7544_7545delCT was performed using conventional PCR-based amplification refractory mutation system (ARMS) method. All 33 samples harboring c.7544_7545delCT allele and 5 negative cases as control were submitted to real-time PCR. Results: Specificity and sensitivity of two PCR techniques were comparable. NOTCH1 c.7544_7545delCT mutation was found by ARMS in 10.1% of CLL patients, which is consistent with the data of other studies. However, the results of ARMS PCR in a minority of cases (2.15%) were doubtful and required reinvestigation. Real-time PCR, being less time-consuming, showed advantage in the assessment of the amplification’s specificity (using the melting curve analysis). It also allows the quantitative assessment of NOTCH1-mutated clone. Conclusion: NOTCH1 c.7544_7545delCT mutation resulting in removal of the C-terminal PEST domain, deregulation of NOTCH1-dependent signaling pathways, has negative influence on prognosis of CLL and efficiency of therapy with anti-CD20 monoclonal antibodies. Real-time PCR allows the fast and reliable detection of c.7544_7545delCT mutation and can be used for the screening of this molecular lesion in CLL patients. Fri, 01 Jan 2016 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138001 2016-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/138000 Establishment and characterization of new breast and ovarian cancer cell lines as a model for studying cellular plasticity in vitro Bezdieniezhnykh, N.; Lykhova, A.; Semesiuk, N.; Okhrimenko, R.; Kudryavets, Yu. Aim: To compare biological properties of primary tumor cells isolated from malignant effusion of cancer patients with the same cells of permanent lines established during their long-term cultivation in vitro and to assess the impact of phenotypic conversion that was caused by changes in their microenvironment on their behavioral characteristics. Materials and Methods: The study was performed on primary cell cultures from pleural effusion or ascites of breast and ovarian cancer and permanent cell lines derived from them, namely permanent ovarian cancer cell line I, permanent ovarian cancer cell line II and permanent breast cancer cell line I. Biological characteristics were studied using standard cell culture methods and immunocytochemical assays. Results: Three new cell lines were established from breast and ovarian cancer and cell morphology, migration activity, the kinetics of growth, colony forming activity in semisolid agar and sensitivity to anticancer drug were examined. These characteristics were compared with those of the primary tumor cells. It has been shown that among the primary tumor cells from malignant effusion, cells with mesenchymal characteristics were the most prevalent. Cultivation of primary cancer cells in vitro leads to a phenotypic change of their population: it becomes more homogeneous in morphology with predominantly epithelial-like cells. Also, later after a number of cell doublings in vitro, the cell population changes to include cells primarily with immunophenotypic properties characteristic of epithelial cells. These changes include increase in number of E-cadherin-positive cells and a decrease in number of vimentin and α- smooth muscle actin-positive cells. It was found that significant changes in expression of epithelial-mesenchymal transition associated proteins in cells during their cultivation in vitro in new microenvironment are accompanied by a rapid change in their sensitivity to anticancer drugs. Conclusions: The new breast and ovarian cancer cell lines were established and characterized. The induction of phenotypic transdifferentiation in malignant cells from pleural effusion and ascites can be an important approach for suppressing the progression of neoplastic process. Fri, 01 Jan 2016 00:00:00 GMT http://dspace.nbuv.gov.ua:80/handle/123456789/138000 2016-01-01T00:00:00Z