http://dspace.nbuv.gov.ua:80/handle/123456789/2903 2026-04-22T22:54:24Z http://dspace.nbuv.gov.ua:80/handle/123456789/3154 Функцiональна характеристика мiкроiнкапсульованої тканини аденоми паращитовидної залози людини в умовах in vitro Пастер, І.П. The microencapsulated tissue of human parathyroid adenoma keeps a high functional activity in vitro (in particular, the ability for active parathormone secretion and an adequate reaction to extracellular calcium) that makes it suitable for using it as a transplant in the compensation of the hypofunctional state of the parathyroid system. 2007-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/3153 Каліксарен біс-гідроксиметилфосфонова кислота змінює механокінетичні параметри скорочення кільцевих гладеньких м’язів caecum щура, що викликані ацетилхоліном Цимбалюк, О.В.; Онуфрийчук, О.В.; Мірошниченко, М.С.; Черенок, С.О.; Кальченко, В.І.; Костерін, С.О. We studied the influence of calixarene bis-hydroxymethylphosphone acid (code C99) on the mechanokinetic parameters of the acetylcholine-evoked contractions of smooth muscles caecum strips of rats. It is established that this substance inhibits the amplitude and selectively modifies the mechanokinetic characteristics of contractions. The kinetic analysis has shown that the normalized maximal rate of contraction was not changed relative to control, and the normalized maximal rate of relaxation was significantly inhibited by calixarene С99 in such a manner that the form of dependence of this parameter on the effector concentration changed from sigmoid (in control) to linear. 2007-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/3152 Клонування та експресія білків Mycobacterium bovis MPB63 і MPB83 у клітинах Escherichia coli Редчук, Т.А.; Олійник, О.С.; Кабернюк, А.А.; Буркальова, Д.О.; Романюк, С.І.; Колибо, Д.В.; Комісаренко, С.В. Gene fragments mpb83 and mpb63 from Mycobacterium bovis BCG were amplified using PCR. Plasmids containing mpb63 and mpb83 fragments have been constructed using the expressi- on vector pET24a (Novagen). Expression of recombinant proteins was obtained in E. coli Rosetta (DE3) сеlls under control of strong T7 promoter. Lysates were analyzed by immu- noblotting with anti HisTag conjugate. His-Tag fusion proteins were purified with batch metal affіnity chromatography under denaturative conditions. Results are expected to lay groundwork for the further development of improved diagnostic tools or vaccines against human and bovine tuberculosis. 2007-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/3151 Системные биохимические нарушения при экспериментальном аллергическом энцефаломиелите Кучмеровская, Т.М.; Гурина, Н.М.; Шиманский, И.А.; Донченко, Г.В.; Супрун, С.М.; Клименко, А.П. Reduced levels of total lipids and cholesterol in blood serum were shown after 10 days of experimental allergic encephalomyelitis (EAE) induction. The elevated content of blood serum phospholipids was observed after 18 days of EAE duration. These alterations were accompanied by the progressive increase in both the total proteolytic activity and acid phosphatase activity with a decreasing effect on alkaline phosphatase in blood serum. Compared to control, the blood content of vitamin B12 was twice lower in the period as early as 10 days after EAE induction. Our findings suggest the presence of system biochemical impairments, which most likely reflect the degree of EAE-related destructive changes in brain and may be of significance as diagnostic and prognostic criteria for multiple sclerosis management. 2007-01-01T00:00:00Z