http://dspace.nbuv.gov.ua:80/handle/123456789/133178 2026-04-06T15:11:47Z http://dspace.nbuv.gov.ua:80/handle/123456789/145710 Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats Chekhun, V.F.; Storchai, D.M.; Todor, I.N.; Borikun, T.V.; Lukianova, N.Yu. Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity. 2018-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/145639 A case report of late local relapse of adrenocortical carcinoma 18 years after adrenalectomy Tkachenko, R.; Golovko, A.; Kuryk, O. Adrenocortical cancer is an extremely rare tumor presenting with extensive locoregional spread at the time of diagnosis. Due to the diagnostic difficulties preoperatively and a lack of effective treatment options, patients have poor prognosis. Patients succumb to metastases within a couple of months. Only 20 cases have been so far reported in the literature with a medium disease-free survival up to 2 years. We present a case of a locoregional recurrence of adrenocortical cancer 18 years after left adrenalectomy. Key Words: аdrenocortical carcinoma, local relapse, adrenalectomy, long-term survival, nephrectomy. 2018-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/145638 ABO blood group and the risk of lung cancer in Greek adults: a case — control study Chrysanthakopoulos, N.A.; Dareioti, N.S. Aim: The present study aimed to investigate any possible association between ABO blood groups and lung cancer. Materials and Methods: The study was conducted on 122 lung cancer patients and 1,255 matched-healthy individuals that were reviewed retrospectively. Chi-square and logistic regression models were used for statistical analysis. Results: No significant difference between lung cancer patients and the control group was recorded regarding ABO blood types and the risk of lung cancer (p = 0.055, OR = 0.79, 95% CI 0.61–1.03). Male gender (p = 0.006, OR = 2.08, 95% CI 1.24–3.49) and smoking (p = 0.000, OR = 3.13, 95% CI 1.72–5.69) were significantly associated with the risk of lung cancer. Conclusion: No association between ABO blood types and the risk of lung cancer was observed. Key Words: ABO blood types, lung cancer, adults, risk factor. 2018-01-01T00:00:00Z http://dspace.nbuv.gov.ua:80/handle/123456789/145637 Markers of the epithelial-mesenchymal transition in cells of endometrial carcinoma Nesina, I.P.; Iurchenko, N.P.; Buchynska, L.G. Aim: To study the expression of adhesion markers (E-cadherin, β-catenin and vimentin) associated with epithelial-mesenchymal transition (EMT) and their role in progression of endometrial carcinoma (EC). Materials and Methods: Expression of E-cadherin, β-catenin and vimentin was studied immunohistochemically in the samples of surgical material of 55 EC patients stage I–III. The proliferation index was determined by flow cytometry. Results: In the group of vimentin-negative EC, tumors of low differentiation grade and deep invasion in myometrium as well as high expression of E-cadherin and β-catenin prevailed compared with the cases with high expression of vimentin. In addition, in EC with high expression of vimentin, an increase in the number of cells with expression of E-cadherin in the cytoplasm (78.9 ± 3.6%) and β-catenin with cytoplasmic-nuclear localization (73.7 ± 3.2%) was observed compared with these indices in vimentin-negative tumors (45.4 ± 4.2%, p < 0.001 and 54.5 ± 2.6%, respectively, p < 0.005), which may indicate EMT-associated changes in EC with high expression of vimentin. Conclusions: The progression of the endometrioid carcinoma may occur in the setting of various molecular changes, in particular, with decreased expression of E-cadherin and β-catenin and high expression of vimentin, or in the absence of vimentin, utilizing other mechanisms of regulation of proliferative and metastatic potential. Key Words: endometrial cancer, proliferation index, E-cadherin, β-catenin, vimentin. 2018-01-01T00:00:00Z