http://dspace.nbuv.gov.ua:80/handle/123456789/2910 2026-04-15T12:15:30Z 2026-04-15T12:15:30Z Руденко, С.С. Зароченцева, О.Д. http://dspace.nbuv.gov.ua:80/handle/123456789/4171 2009-12-11T15:23:56Z 2008-01-01T00:00:00Z

Вплив iмiтованих кислотних дощiв на продуктивнiсть лiсових екосистем у мiкрокосмах Руденко, С.С.; Зароченцева, О.Д. The influence of a simulated acid rain with pH = 2.0 and pH = 3.0 on the biomass increment and its structure is investigated for main forest-forming species of the Chernivtsi region in microcosmical models. A decrease of the biomass increment for all species under the action of an acid rain with pH = 2.0 is shown. The influence of an acid rain with pH = 3.0 stimulates the biomass increment in conifers.

2008-01-01T00:00:00Z Захарченко, Т.Ф. Замотаєва, Г.А. Тронько, М.Д. http://dspace.nbuv.gov.ua:80/handle/123456789/4170 2009-07-22T09:00:55Z 2008-01-01T00:00:00Z

Порiвняльний аналiз цитотоксичностi природних клiтин-кiлерiв у хворих з доброякiсними та злоякiсними пухлинами щитовидної залози до та пiсля лiкування Захарченко, Т.Ф.; Замотаєва, Г.А.; Тронько, М.Д. The authors have studied the cytotoxic activity of natural killer (NK) cells. With this purpose, the NK-cell activity was determined in 49 patients with benign thyroid tumours and in 48 patients with malignant thyroid tumours (aged from 18 to 65 years, 84 women and 56 men) depending upon the age, gender, degree of spreading, and presence of tumour metastases before surgery and in 1 to 6 months after surgery. The control group (43 subjects) consisted of practi- cally healthy persons of appropriate age and gender. In a second series of investigations, the authors have examined 35 patients with papillary thyroid cancer (TC) before prescribing a regular course of radioiodine therapy and 25 patients from this group who had received radi- oiodine therapy for eliminating a residual thyroid tissue and treating metastases. It has been shown that the NK-cell cytotoxic activity in patients with TC did not depend upon the age, but depended to a large extent upon the gender, degree of tumour spreading, and presence of metastases, being the lowest at T4 stage and in the group with metastases. Unlike pati- ents with benign tumours, in patients with papillary TC, the NK-cell activity was significantly suppressed. After surgical treatment of patients with TC, the NK-cell activity was decreased by 1.5 times as compared to that in healthy donors. After radioiodine therapy, the NK-cell activity in patients with papillary TC was additionally decreased by 1.3 times as compared to the initial indicator.

2008-01-01T00:00:00Z Кабернюк, А.А. Олійник, О.С. Редчук, Т.А. Романюк, С.І. Колибо, Д.В. Комісаренко, С.В. http://dspace.nbuv.gov.ua:80/handle/123456789/4169 2018-03-12T13:12:21Z 2008-01-01T00:00:00Z

Клонування генів рекомбінантних субодиниць дифтерійного токсину Corynebacterium diphtheriae та їх експресія в клітинах Escherichia coli Кабернюк, А.А.; Олійник, О.С.; Редчук, Т.А.; Романюк, С.І.; Колибо, Д.В.; Комісаренко, С.В. The diphtheria toxin is the main pathogen of Corynebacterium diphtheriae. This is a protein with molecular weight 58,360. Having penetrated into endosomes of sensitive cells, diphtheria toxin is digested into two subunits: A and B. Fragments of diphtheria toxin’s gene which encode subunits A and B have been amplified from genomic DNA of Corynebacterium diphtheriae strain NCTC 10648. Plasmids containing subunit A and subunit B have been constructed using expression vector pET24a. Expression of recombinant subunits was performed in E. coli strain Rosetta (DE3) сеlls under control of strong T7 promoter. Recombinant proteins were purified from cell lysate under denaturating conditions by affinity chromatography. Antigen properties were confirmed by western blot analysis.

2008-01-01T00:00:00Z Нагорний, П. Г. Корнієнко, З.І. Бойко, Р.С. Городилова, Н.О. Слободяник, М.С. http://dspace.nbuv.gov.ua:80/handle/123456789/4168 2018-03-12T13:27:12Z 2008-01-01T00:00:00Z

Синтез подвійних фосфатофторидів лужних металів та нікелю (II) Нагорний, П. Г.; Корнієнко, З.І.; Бойко, Р.С.; Городилова, Н.О.; Слободяник, М.С. Complex fluorophosphates of nickel and alkali elements M2NiPO4F (M = Li, Na) have been synthesized from flux melts of the system of fluorophosphates. The synthesis conditions and the existence area of synthesized phosphates are pointed out. The obtained compounds are investigated by X-ray FA, IR spectroscopy and quantitative analyses.

2008-01-01T00:00:00Z