http://dspace.nbuv.gov.ua:80/handle/123456789/161349 2026-04-06T10:18:26Z 2026-04-06T10:18:26Z Хохлов, А.М. http://dspace.nbuv.gov.ua:80/handle/123456789/178356 2021-02-18T23:27:47Z 2014-01-01T00:00:00Z

Эволюция зубной и костной системы у представителей рода Sus Хохлов, А.М. Aims. Questions about phylogenetic development of tooth system in Sus gender representatives and also osteogenesis and processes in ontogenesis of wild European boar and domesticated breed of White Swine haven’t been enough covered in scientific sources. Methods. Morphological, zootechnical and histological methods of investigation of separate skeletal bones formation in ontogenesis of wild boar and White Swine breed have been used. Results. Weight of bone and osteogenesis in foetus of wild European boar in comparison with foetus development of White Swine breed has been studied. There was boar’s leg weight during 50-days age more on 118 %, than White Swine’s one. Bones of domestic animals grew more intensively at 70-days age that is connected with domestication process. However, development of red marrow and erithropoesis took place more intensively in bony tissue of wild animals. Conclusions. Premolars and incisors of Sus gender have been the most changed during evolutional processes of tooth system. Formation of bone and muscle system in ontogenesis process of wild and domestic swine proved that there was high intensity of osteogenesis for domestic animals, and there was high intensity of erithropoesis for wild swine. Wild boar can be donor of valuable peculiarities for high natural resistance and life of modern breeds. Key words: genus Sus, evolution, tooth and bone systems.

2014-01-01T00:00:00Z Танасієнко, І.В. Бузіашвілі, Н. Ємець, А.І. Блюм, Я.Б. http://dspace.nbuv.gov.ua:80/handle/123456789/178355 2021-02-19T23:27:02Z 2014-01-01T00:00:00Z

Агробактеріальна трансформація томатів (Solanum lycopersicon) геном лактоферину людини Танасієнко, І.В.; Бузіашвілі, Н.; Ємець, А.І.; Блюм, Я.Б. Aims. Chemicals are classical agents for the control of plant diseases, however, the emergence of new resistant pathogens strains and their toxins as well as chemicals’ limited biological activity and negative long-term effects both on human health and the environment are the challenges for biotechnologists to develop an alternative approach of plant protection. Methods. Cell and tissue culture protocols were combined with Agrobacterium-mediated transformation to obtain modified tomato plants. Results. A range of explants, culture mediums, pre-cultivation time and their combination were established for the development of the effective tomato transformation protocol. Therefore, the regeneration of green shoots from the transformed explants under the selective pressure has been obtained. Conclusions. The effective protocol for transformation of commercial tomato cultivar “Many maker” was obtained. Key words: Agrobacterium-mediated transformation, tomato, human lactoferrin gene, systemic resistance.

2014-01-01T00:00:00Z Моргун, Б.В. Чугункова, Т.В. Лялько, І.І. Великожон, Л.Г. Степаненко, А.І. http://dspace.nbuv.gov.ua:80/handle/123456789/178354 2021-02-18T23:27:44Z 2014-01-01T00:00:00Z

Молекулярно-генетична ідентифікація та цитологічні особливості сортів озимої м’якої пшениці з пшенично-житньою транслокацією Моргун, Б.В.; Чугункова, Т.В.; Лялько, І.І.; Великожон, Л.Г.; Степаненко, А.І. Aims. To reveale the presence of wheat-rye translocations in the genome of varieties of soft winter whea by PCR-analysis using primers to locus Xrems 1303 and ω-secalin located in the short arm of chromosome 1R of rye and cytological methods. Methods. DNA analysis by polymerase chain reaction and electrophoretic determination of amplification products. The methods of meios analysis. Results. Varieties of soft winter wheat with wheat-rye translocation were discovered. Conclusions. Specific primers to locus located in chromosome arm 1RS of rye can be used to analyze the presence in wheat wheat-rye translocation. The presence of wheat-rye translocation are confirmed by cytological analysis. Key words: Triticum aestivum L., DNA markers, PCR-analysis, multiplex-PCR, rye-wheat translocation, cytological methods.

2014-01-01T00:00:00Z Маменко, А.М. Емец, З.В. Антоненко, С.Ф. http://dspace.nbuv.gov.ua:80/handle/123456789/178353 2021-02-18T23:27:50Z 2014-01-01T00:00:00Z

Наследуемость и изменчивость уровня содержания и выхода молочного жира как селекционных признаков в стадах черно-пестрого скота Маменко, А.М.; Емец, З.В.; Антоненко, С.Ф. Aims. According to some researchers, one of the main quality of the show-of-milk – fat – changed under the influence of various factors, the main of which are genetic. Methods. Laboratory of physico-chemical tests of an experimental material with use of the method of atomic-absorption spectrophotometry ААS-30 (Carl Zeiss, Jena), held – biometric processing of the received results. Results. It is established that increasing the fat content of the milk of the mother at the 1 % growth can be expected to fat content in milk daughter 0.29 %. The degree of influence of the factor «Father» on the fat content in milk amounted to 0.127, and the output of milk fat 0,196 at high statistical reliably-STI (P > 0.999). Conclusions. When increasing the fat content in the milk of the mother of 1% can be expected growth of this indicator for the daughters of 0.29 %. Correlation between exposure-house milk fat mothers and daughters reaches r = 0.28 (P > 0.999). Coefficients " you heritability of fat content in milk and milk output fat are respectively 0,56 and 0.72. The most significant of the genetic factor is the father. By this factor may describe 12.7 % of the variance of with-holding of fat in milk and 19.6 % of the variance of output of milk fat. There joint influence of the factor «father» to the other: «the father year calving» (η2 = USD 0.087 on the fat content and η2 = 0.11 per output of milk fat), «the father hundred-up» (η2 = 0.065, and η2 = 0.142 respectively). Key words: milk of cows, genetically, the reliability of the impact, mother, father.

2014-01-01T00:00:00Z