Experimental Oncology, 2016 (том 38)

Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus

2018-06-23T00:03:13Z

Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus Lykhova, O.; Kovalova, O.; Bezdenezhnykh, N.; Adamenko, I.; Vorontsova, A.; Strokovska, L.; Kudryavets, Yu. Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguﬁcant decrease of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene signiﬁcantly inhﬂtited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused signiﬁcant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in signiﬁcant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed expression ofproteins associated with EMT.

miR-608 rs4919510 C>G polymorphism decreased the risk of breast cancer in an Iranian subpopulation

2018-06-23T00:03:13Z

miR-608 rs4919510 C>G polymorphism decreased the risk of breast cancer in an Iranian subpopulation Hashemi, M.; Sanaei, S.; Rezaei, M.; Bahari, G.; Hashemi, S.M.; Mashhadi, M.A.; Taheri, M.; Ghavami, S. Aim: MicroRNAs (miRNAs) are small noncoding RNAs that function as oncogene or tumor suppressors. The single nucleotide polymorphisms in miRNAs potentially can alter miRNA-binding sites on target genes as well as affecting miRNAs expression. The present study aimed to evaluate the impact of miR-608 rs4919510 C>G variant on breast cancer (BC) risk. Materials and Methods: This case-control study conducted on 160 women with BC and 192 age-matched healthy women. Genotyping of miR608 rs4919510 was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Our findings showed that GC genotype significantly decreased the risk of BC (odds ratio (OR) = 0.49, 95% confidence interval (CI) 0.28–0.88, p = 0.018) compared to CC genotype. Furthermore the G allele decreased the risk of BC (OR = 0.53, 95%CI 0.30–0.92, p = 0.024). No significant association was found between miR-609 genotypes and clinicopathological characteristics of BC patients (p > 0.05). Conclusion: Our findings indicate that miR-608 polymorphism might be associated with decreased risk of BC in an Iranian subpopulation. Further large-scale studies with different ethnicities are needed to verify our findings.

The III Swedish-Ukrainian meeting on cancer diseases

2018-06-23T00:03:03Z

The III Swedish-Ukrainian meeting on cancer diseases

Radioprotective properties of sodium humate in radiation-induced mutagenesis in cultured lymphocytes of thyroid cancer patients

2018-06-18T00:05:20Z

Radioprotective properties of sodium humate in radiation-induced mutagenesis in cultured lymphocytes of thyroid cancer patients Shkarupa, V.M.; Klymenko, S.V. Aim: To investigate the effect of sodium humate on the level of cytogenetic damage in culture of lymphocytes of patients with thyroid cancer after γ-irradiation. Materials and Methods: Metaphase analysis of chromosome aberrations in cultured peripheral blood lymphocytes of 10 individuals with thyroid cancer was performed after irradiation of lymphocytes in vitro at a dose of 1 Gy from ¹³⁷Cs source at the early G₀ phase of cell cycle. Sodium humate was added to cell culture for 30 ± 15 min after phytohemagglutinin stimulation at concentrations of 10 and 100 μg/ml. Results: Sodium humate exhibited antimutagenic properties. The preparation at a concentration of 10 μg/ml was more effective than at a concentration of 100 μg/ml, reducing the average incidence of radiation-induced chromosome aberrations by 51.88 and 38.77%, respectively. The most pronounced antimutagenic effect of sodium humate was the reduction of the frequency of chromosomal type aberrations, however, such efficiency varied between individual patients with thyroid cancer. Conclusions: Sodium humate could be considered as a potential therapeutic modifier of radiation damage.