http://dspace.nbuv.gov.ua:80/handle/123456789/133130 2026-04-18T11:34:19Z 2026-04-18T11:34:19Z Kopczynska, E. Dancewicz, M. Kowalewski, J. Makarewicz, R. Kardymowicz, H. Kaczmarczyk, A. Tyrakowski, T. http://dspace.nbuv.gov.ua:80/handle/123456789/138724 2018-06-20T00:06:31Z 2012-01-01T00:00:00Z

Influence of surgical resection no plasma Endoglin (CD105) level in non-small cell lung cancer patients Kopczynska, E.; Dancewicz, M.; Kowalewski, J.; Makarewicz, R.; Kardymowicz, H.; Kaczmarczyk, A.; Tyrakowski, T. Background and Aim: Endoglin is a proliferation-associated antigen on endothelial cells and essential for angiogenesis. Soluble endoglin (s‑endoglin), formed by proteolytic cleavage of ectodomain of membrane receptor could be an indicator of tumor‑activated endothelium. The aim of present study was to analyze changes of s‑endoglin level in plasma of lung cancer patients following surgical resection and to estimate the correlation of s‑endoglin with other soluble receptors, sTie2 and sVEGF R1. Patients and Methods: The study group consisted of 37 patients with stage I of non-small cell lung cancer. Plasma concentrations of s‑endoglin, sTie2 and sVEGF R1 were evaluated by ELISA, three times: before surgical resection and on postoperative day 7 and 30. Results: The median of s‑endoglin concentration decreased significantly on postoperative day 7 when compared with preoperative level and next increased on 30th day and it was comparable with that before surgery. s-Endoglin correlated with another soluble receptors, with sTie2 both before surgery (r=0.44) and on postoperative day 7 (r=0.52) and on 30th day (r=0.58), with sVEGF R1 — only on postoperative day 7 (r=0.75). Conclusion: The increased level of serum endoglin in lung cancer patients compared to controls and its changes after surgical treatment suggest potential application of soluble form of endoglin as potential tumor marker.

2012-01-01T00:00:00Z Berzina, D. Irmejs, A. Kalniete, D. Borosenko, V. Nakazawa-Miklasevica, M. Ribenieks, K. Trofimovics, G. Gardovskis, J. Miklasevics, E. http://dspace.nbuv.gov.ua:80/handle/123456789/138723 2018-06-20T00:06:00Z 2012-01-01T00:00:00Z

Novel germline MLH1 and MSH2 mutations in latvian Lynch syndrome families Berzina, D.; Irmejs, A.; Kalniete, D.; Borosenko, V.; Nakazawa-Miklasevica, M.; Ribenieks, K.; Trofimovics, G.; Gardovskis, J.; Miklasevics, E. Background/Aims: Hereditary non-polyposis colorectal cancer or Lynch syndrome is an autosomal dominantly inherited disease with high penetrance, mostly due to mutations in the MLH1 and MSH2 genes. The aim of this study is to investigate the mutation spectrum of the MLH1 and MSH2 genes. Methodology: High risk colorectal cancer families were selected from overall 1053 consecutive patients. Screening of germline mutations in the MLH1 and MSH2 was performed by direct sequencing and multiplex ligation-dependent probe amplification. Results: Ten patients fulfilled the Amsterdam I/II criteria and Bethesda guidelines of the Lynch syndrome. Three novel mutations were identified in MLH1 and MSH2 genes, as well as two known mutations in the MLH1 gene. Large rearrangements in the MLH1 gene were found in two patients. Conclusions: The mutations in the MLH1 and MSH2 genes in Latvian high-risk families are highly heterogeneous. Combination of direct sequencing and MLPA is the most appropriate molecular method of detecting hereditary nonpolyposis colorectal cancer patients and family members at risk.

2012-01-01T00:00:00Z Nikbakht, M. MalekZadeh, K. Kumar Jha, A. Askari, M. Marwaha, R.K. Kaul, D. Kaur, J. http://dspace.nbuv.gov.ua:80/handle/123456789/138722 2018-06-20T00:05:57Z 2012-01-01T00:00:00Z

Polymorphisms of MTHFR and MTR genes are not related to susceptibility to childhood ALL in north India Nikbakht, M.; MalekZadeh, K.; Kumar Jha, A.; Askari, M.; Marwaha, R.K.; Kaul, D.; Kaur, J. Background:Acute lymphoblastic leukemia (ALL) is the most worldwide common type of childhood cancer. Methylenetetrahydrofolate reductase (MTHFR) and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) participate in folate pathways and are known as critical factors for DNA integrity as well as DNA hypomethylation. The aim of this work is to investigate frequency of MTHFR (677C→T and 1298A→C) and MTR (2756A→G) polymorphisms and their interaction with respect to possible effect on risk of childhood ALL among North Indian population. Procedure: A case control study from has been conducted on bone marrow and peripheral blood samples from 125 ALL patients and 100 sex-age matched healthy controls using PCR-RFLP method. Results: No statistically significant differences were observed for different genotypes between patients and controls (p>0.05). Significant difference for the risk of ALL in individuals having genotype of MTHFR 677TT (OR=0.61, 95% CI=0.21–1.77) and MTHFR 1298CC (OR=0.56, 95% CI=0.18–1.68) was not observed. The correlation of SNP of MTR gene and risk of ALL was not observed, too. Conclusions: The differences in distribution of possible combined genotypes of MTHFR (677C→T, 1298A→C) and MTR (2756A→G) between ALL patients and controls were statistically insignificant.

2012-01-01T00:00:00Z Gerashchenko, B.I. Ryabchenko, N.M. Glavin, O.A. Mikhailenko, V.M. http://dspace.nbuv.gov.ua:80/handle/123456789/138721 2018-06-20T00:06:32Z 2012-01-01T00:00:00Z

The long-range cytotoxic effect in tumor-bearing animals Gerashchenko, B.I.; Ryabchenko, N.M.; Glavin, O.A.; Mikhailenko, V.M. Aim:The relationship between cancer and patient health is still of great interest for experimental and clinical oncology. The tumor can adversely affect surrounding and distant tissues as well. However, effects of the tumor on distant tissues are much less studied than its effects on surrounding tissues. This study was aimed to test whether the tumor could trigger cytotoxic and/or genotoxic signals with respect to the distant proliferative tissue such as bone marrow. Materials and Methods: Rats were subcutaneously implanted with Guerin carcinoma cells, and on the 12th and 18th days after implantation both cytotoxic and genotoxic effects were assessed by flow cytometry in acridine orange stained unfractionated bone marrow cells isolated from femur. The cytotoxic effect was assessed using ratios of the following cell populations: total nucleated cells (TNC)/total enucleated erythrocytes (TE); polychromatic erythrocytes (PCE)/normochromatic erythrocytes (NCE). The genotoxic effect was assessed by quantification of micronucleated PCE (MNPCE) within the population of PCE. Results: A significant cytotoxic effect was observed in tumor-bearing animals on the 12th and 18th days after implantation (≈ 2-fold decrease in both TNC/TE and PCE/NCE ratios compared with corresponding parameters in control animals). There was also a genotoxic effect in these animals (a slight increase in the number of MNPCE), however, this effect was insignificant. The PCE/NCE ratio reversely correlated with the tumor weight which is suggestive of the link between erythropoietic cytotoxicity and tumor progression. Conclusion: Cytotoxic insult to the bone marrow is likely to be associated with the mechanism(s) triggered by distantly located tumors whose growth may correlate with the cytotoxic effect.

2012-01-01T00:00:00Z